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KMID : 0370219970410060692
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Yakhak Hoeji
1997 Volume.41 No. 6 p.692 ~ p.697
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Determination of 3 , 4-Dimethylhippuric Acid and Creatinine from the Urine of Workers Exposed to 1 , 2 , 4-Trimethylbenzene
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임정미/Lim JM
장재연/이용문/강종성/Jang JY/Lee YM/Kang JS
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Abstract
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Creatine and 3.4-Dimethylhippuric acid (3,4-DMHA), a glycine conjugate of 1.2,4-trimethylbenzene (1,2,4-TMB) were determined in the urine of workers exposed to 1,2,4-TMB vapor. The best condition for the simultaneous determination of 3.4-DMHA and creatine by high performance liquid chromatography was obtained by reverse phase C18 column (4.6X150mm, 5mcm) as stationary phase and 20% acetonitrile in 20mM phosphate buffer (pH 3.0) containing 4mM sodium octylsulfate(SOS)as mobile phase. The recovery of 3,4-DMHA spiked to blank urine in the range of 1~5mcg/ml was about 96%. The concentration of urinary 3,4-DMHA of workers had a positive correlation with the environmental level of 1,2.4-TMB (r=0.866). The data suggest that urinary 3,4-DMHA concentration is a useful biological index for 1,2.4-TMB exposure.
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KEYWORD
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